Identification of androgen receptors in normal human osteoblast-like cells.
نویسندگان
چکیده
منابع مشابه
Identification of androgen receptors in normal human osteoblast-like cells.
The sex steroids, androgens and estrogens, are major regulators of bone metabolism. However, whether these hormones act on bone cells through direct or indirect mechanisms has remained unclear. A nuclear binding assay recently used to demonstrate estrogen receptors in bone [Eriksen, E.F., Colvard, D.S., Berg, N.J., Graham, M.L., Mann, K.G., Spelsberg, T.C. & Riggs, B.L. (1988) Science 241, 84-8...
متن کاملFormation in Isolated Human Osteoblast-Like Cells
The effect of bradykinin on prostaglandin E 2 formation in cells from human trabecular bone has been studied. The cells responded to parathyroid hormone with enhanced cyclic AMP formation and were growing as cuboidal-shaped, osteoblast-like cells. In these isolated human osteoblast-like cells, bradykinin (1 #tool/l) caused a rapid (5 min) stimulation of prostaglandin E2 formation. This finding ...
متن کاملHuman osteoblast-like cells contain specific, saturable, high-affinity glucocorticoid, androgen, estrogen, and 1 alpha,25-dihydroxycholecalciferol receptors.
Glucocorticoids, androgens, estrogens and 1 alpha,25-dihydroxycholecalciferol (1 alpha,25-(OH)2D3) exert a variety of effects on bone homeostasis. We have measured the receptors for these hormones in cultured human osteoblast-like cells. Specific binding was found with each of the four steroids tested, namely maximum binding capacities, Bmax, of 2,581 (1,368-4,223), 146 (101-237), 176 (20-552),...
متن کاملResponses of human normal osteoblast cells and osteoblast-like cell line, MG-63 cells, to pulse electromagnetic field (PEMF)
The objective of this in vitro study is to investigate the effect of pulsed electromagnetic field (PEMF) on cellular proliferation and osteocalcin production of osteoblast-like cell line, MG-63 cells, and human normal osteoblast cells (NHOC) obtained from surgical bone specimens. The cells were placed in 24-well culture plates in the amount of 3x104 cell/wells with 2 ml αMEM media supplemented ...
متن کاملLigand-induced downregulation of receptors for TGF-beta in human osteoblast-like cells from adult donors.
High concentrations of transforming growth factor b (TGF-beta) are found in the bone matrix, reflecting a pivotal role of this growth factor in the coupling of bone resorption and formation. TGF-beta strongly stimulates the synthesis of extracellular matrix proteins, but in vitro studies show an inhibitory effect on the final mineralization process, which in vivo occurs despite high concentrati...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1989
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.86.3.854